Coding

Part:BBa_K642005:Design

Designed by: Wilson Lam, Matthew Orton   Group: iGEM11_uOttawa   (2011-09-23)

LacI repressor tagged with yBFP and a VP16 activation domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1084
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Both LacI and yeast codon optimized BFP were designed without a stop codon to enable the construction of a fusion protein with BFP and the rtTA activation domain.

Source

The LacI repressor was PCR amplified from a plasmid source of a biobrick (BBa_K319007) that was submitted last year by the our team. Yeast codon optimized BFP was synthesized by the uOttawa team using Mr. Gene DNA synthesis. The VP16 activation domain was PCR amplified from a plasmid used by our lab called pDVA38.

References